PDE4A Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89217A
Western blot analysis of extracts of various cell lines using PDE4A Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat kidney using PDE4A Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using PDE4A Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of K-562 cells using PDE4A Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using PDE4A Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using PDE4A Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
PDE4A Polyclonal Antibody
Catalog Number
RD89217A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human PDE4A
Gene ID
5141
Swissprot
P27815
Synonyms
PDE4DPDE2PDE46
Calculated MW
14kDa
Observed MW
14KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas