PDHA1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD252930A
Western blot analysis of A549 Hela and HepG2 cell lysates using PDHA1 Polyclonal Antibody at dilution of 1:750
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using PDHA1 Polyclonal Antibody at dilution of 1:50(×200)
Product Name
PDHA1 Polyclonal Antibody
Catalog Number
RD252930A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
PDHA1(Pyruvate dehydrogenase E1 component subunit alpha,somatic form,mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA.It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).
Immunogen Information
Immunogen
Fusion protein of human PDHA1
Swissprot
P08559
Synonyms
ODPAPDHPDHAPDHA1PDHCE1APDHE1 A type IPDHE1-A type IPHE1APyruvate Dehydrogenase (lipoamide) alpha 1Pyruvate dehydrogenase complexE1 alpha polypeptide 1Pyruvate Dehydrogenase E1 alphaPyruvate dehydrogenase E1 component subunit alphasomatic form
Calculated MW
43 kDa
Observed MW
Refer to figures
Gene Accession
BC002406
Applications
Reactivity
Human, Mouse, Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:1000-1:5000, IHC 1:50-1:300, ELISA 1:5000-1:10000
Concentration
0.9 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas