PDK1/PDHK1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87122A
Western blot analysis of extracts of various cell lines using PDK1/PDHK1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of HepG2 cells using PDK1/PDHK1 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of NIH/3T3 cells using PDK1/PDHK1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using PDK1/PDHK1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
PDK1/PDHK1 Polyclonal Antibody
Catalog Number
RD87122A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human PDK1/PDHK1
Gene ID
5163
Swissprot
Q15118
Synonyms
PDK1
Calculated MW
53kDa/59kDa/60kDa
Observed MW
75kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas