For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88827A
Western blot analysis of extracts of various cell lines using PDXP Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Mouse kidney using PDXP Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat lung using PDXP Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human colon using PDXP Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of L929 cells using PDXP Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Pyridoxal 5-prime-phosphate (PLP) is the active form of vitamin B6 that acts as a coenzyme in maintaining biochemical homeostasis. The preferred degradation route from PLP to 4-pyridoxic acid involves the dephosphorylation of PLP by PDXP.
Immunogen Information
Immunogen
A synthetic peptide of human PDXP
Gene ID
57026
Swissprot
Q96GD0
Synonyms
CINPLPdJ37E16.5PDXP
Calculated MW
60kDa/66kDa
Observed MW
70KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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