C57BL/6 murine splenocytes are stained with Anti-Human/Mouse/Rat CD47 Monoclonal Antibody(PE Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.
CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.