PEMT Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of 293T and 231 cell lysates  using PEMT Polyclonal Antibody at dilution of 1:800
Western blot analysis of 293T and 231 cell lysates using PEMT Polyclonal Antibody at dilution of 1:800
Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue  using PEMT Polyclonal Antibody at dilution of 1:55(×200)
Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using PEMT Polyclonal Antibody at dilution of 1:55(×200)
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

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Product Name
PEMT Polyclonal Antibody
Catalog Number
RD218144A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit

Background

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Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Immunogen Information

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Immunogen
Synthetic peptide of human PEMT
Swissprot
Q9UBM1
Synonyms
PEAMTPEMPTPEMTPEMTPEMT2phosphatidylethanolamine N methyltransferasePhosphatidylethanolamine N-methyltransferasePNMT
Calculated MW
22 kDa
Observed MW
Refer to figures
Gene Accession
NP009100

Applications

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Reactivity
Human, Mouse
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:1000-1:5000, IHC 1:50-1:300, ELISA 1:5000-1:10000
Concentration
1.56 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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