PGAM5 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88446A
Western blot analysis of various lysates using PGAM5 Polyclonal Antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using PGAM5 Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using PGAM5 Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human tonsil using PGAM5 Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of MCF7 cells using PGAM5 Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
PGAM5 Polyclonal Antibody
Catalog Number
RD88446A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.
Immunogen Information
Immunogen
Recombinant fusion protein of human PGAM5
Gene ID
192111
Swissprot
Q96HS1
Synonyms
PGAM5BXLBV68
Calculated MW
28kDa
Observed MW
28kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.