PHKB Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using PHKB Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using PHKB Polyclonal Antibody at dilution of 1:1000.
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
PHKB Polyclonal Antibody
Catalog Number
RD84864A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human PHKB (NP_000284.1).
Gene ID
5257
Swissprot
Q93100
Synonyms
PHKB
Calculated MW
123 kDa/124 kDa
Observed MW
125 kDa

Applications

Circle
Reactivity
Human,Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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