Phospho-AKT1 (Ser473) Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western Blot analysis of PC3 cells using Phospho-AKT1 (Ser473) Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human lung carcinoma tissue using Phospho-AKT1 (Ser473) Monoclonal Antibody at dilution of 1:200.
Product Name
Phospho-AKT1 (Ser473) Monoclonal Antibody
Catalog Number
RD86450A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Immunogen Information
Immunogen
Synthetic Peptide of Phospho-Akt (S473)
Swissprot
P31749
Synonyms
AKT 1AKTAKT1AKT1MGC99656PKBPKB-ALPHAPRKBAProtein Kinase B AlphaProtein kinase BProto-oncogene c-AktRAC AlphaRACRAC-alpha serine/threonine-protein kinaseRAC-PK-alpha
Observed MW
60 kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-p
Conjugation
Unconjugated
Dilution
WB 1:1000-2000, IHC 1:100-200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.