Phospho-Catenin beta (Tyr654) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86081A
Western Blot analysis of 293T cells with Phospho-Catenin beta (Tyr654) Polyclonal Antibody
Product Name
Phospho-Catenin beta (Tyr654) Polyclonal Antibody
Catalog Number
RD86081A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Immunogen Information
Immunogen
Synthesized peptide derived from human Catenin-β around the phosphorylation site of Tyr654
Swissprot
P35222
Synonyms
Beta cateninBeta-cateninCadherin associated proteinCatenin (cadherin associated protein)beta 188kDaCatenin beta 1Catenin beta-1CATNBCHBCATCTNB1CTNNBCTNNB1DKFZp686D02253FLJ25606FLJ37923OTTHUMP00000162082OTTHUMP00000165222OTTHUMP0000016522
Calculated MW
85 kDa
Observed MW
75 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:40000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.