Phospho-CHEK2 (Thr68) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85823A
Western Blot analysis of Hela cells using Phospho-CHEK2 (Thr68) Polyclonal Antibody at dilution of 1:500
Immunohistochemistry of paraffin-embedded Human uterus tissue using Phospho-CHEK2 (Thr68) Polyclonal Antibody at dilution of 1:200
Immunofluorescence analysis of Rat lung tissue using Phospho-CHEK2 (Thr68) Polyclonal Antibody at dilution of 1:200
Product Name
Phospho-CHEK2 (Thr68) Polyclonal Antibody
Catalog Number
RD85823A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
Immunogen Information
Immunogen
Synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68
Swissprot
O96017
Synonyms
CDS 1Cds1Cds1 homologCheckpoint kinase 2Checkpoint like protein CHK2CHEK 2Chek2Chk 2CHK2 checkpoint homolog (S. pombe)CHK2 checkpoint homologCHK2hCds1HuCds 1LFS 2LFS2PP1425RAD 53RAD53Rad53 homologSerine/threonine protein kinase Chk2Ser
Calculated MW
61 kDa
Observed MW
61 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:10000-1:20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas