Phospho-CREB1 (Ser133) Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD85827A
Western Blot analysis of various cells using Phospho-CREB1 (Ser133) Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of various cells using Phospho-CREB1 (Ser133) Polyclonal Antibody at dilution of 1:1000
Immunohistochemistry of paraffin-embedded Human uterus tissue  using Phospho-CREB1 (Ser133) Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Human uterus tissue using Phospho-CREB1 (Ser133) Polyclonal Antibody at dilution of 1:200
Immunofluorescence analysis of Mouse lung tissue using Phospho-CREB1 (Ser133) Polyclonal Antibody at dilution of 1:200
Immunofluorescence analysis of Mouse lung tissue using Phospho-CREB1 (Ser133) Polyclonal Antibody at dilution of 1:200
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Overview

Circle
Product Name
Phospho-CREB1 (Ser133) Polyclonal Antibody
Catalog Number
RD85827A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.

Immunogen Information

Circle
Immunogen
Synthesized peptide derived from human CREB-1 around the phosphorylation site of Ser133
Swissprot
P16220
Synonyms
Active transcription factor CREBcAMP response element binding protein 1cAMP response element binding proteincAMP responsive element binding protein 1cAMP-responsive element-binding protein 1CREBCREB-1CREB1CREB1Cyclic AMP-responsive element-bindin
Calculated MW
37 kDa
Observed MW
37 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300, ELISA 1:5000-1:20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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