Phospho-FOXO3 (Ser253) Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86124A
Western Blot analysis of Hela cells using Phospho-FOXO3 (Ser253) Polyclonal Antibody at dilution of 1:2000
Western Blot analysis of Hela cells using Phospho-FOXO3 (Ser253) Polyclonal Antibody at dilution of 1:2000
Immunohistochemistry of paraffin-embedded Rat testis tissue  using Phospho-FOXO3 (Ser253) Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Rat testis tissue using Phospho-FOXO3 (Ser253) Polyclonal Antibody at dilution of 1:200
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Overview

Circle
Product Name
Phospho-FOXO3 (Ser253) Polyclonal Antibody
Catalog Number
RD86124A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.

Immunogen Information

Circle
Immunogen
Synthesized peptide derived from human FoxO3A around the phosphorylation site of Ser253
Swissprot
O43524
Synonyms
AF6q21AF6q21 proteinDKFZp781A0677FKHR2FKHRL 1FKHRL1FKHRL1P2Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1Forkhead box O3Forkhead box O3AForkhead box protein O3Forkhead box protein O3AForkhead Drosophila homolog of in rhabdomyosarcoma
Calculated MW
71 kDa
Observed MW
97 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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