Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85876A
Western Blot analysis of COS-7 cells with Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody
Immunohistochemistry of paraffin-embedded Mouse kidney tissue using Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody at dilution of 1:200
Immunofluorescence analysis of Rat spleen tissue using Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody at dilution of 1:200
Product Name
Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody
Catalog Number
RD85876A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Immunogen Information
Immunogen
Synthesized peptide derived from human IκB-α around the phosphorylation site of Ser32/36
Swissprot
P25963
Synonyms
I kappa B alphaI-kappa-B-alphaIkappaBalphaIkB-alphaIKBAIKBAIKBalphaMAD 3MAD3Major histocompatibility complex enhancer-binding protein MAD3NF kappa B inhibitor alphaNF-kappa-B inhibitor alphaNFKBINFKBIANuclear factor of kappa light chain gene
Calculated MW
36 kDa
Observed MW
34 kDa
Applications
Reactivity
Human,Mouse,Rat,Monkey
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.