Phospho-IRS1 (Ser636) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85874A
Western Blot analysis of SH-SY5Y cells using Phospho-IRS1 (Ser636) Polyclonal Antibody at dilution of 1:2000
Product Name
Phospho-IRS1 (Ser636) Polyclonal Antibody
Catalog Number
RD85874A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Immunogen Information
Immunogen
Synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser636
Swissprot
P35568
Synonyms
HIRS 1HIRS1Insulin receptor substrate 1IRS 1IRS-1IRS1IRS1OTTHUMP00000164234
Calculated MW
132 kDa
Observed MW
150 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.