Phospho-p70 S6 kinase alpha (Ser418) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86195A
Western Blot analysis of HT-29 serum 20% 15' cells using Phospho-p70 S6 kinase alpha (Ser418) Polyclonal Antibody at dilution of 1:1000
Product Name
Phospho-p70 S6 kinase alpha (Ser418) Polyclonal Antibody
Catalog Number
RD86195A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
P70 S6 Kinase alpha is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and contains 2 non-identical kinase catalytic domains. It phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein is activated by serine/threonine phosphorylation and protein kinase C and inactivated by type 2A phosphatase. Activation leads to an increase in protein synthesis and cell proliferation. Overexpression of this kinase is involved in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.
Immunogen Information
Immunogen
Synthesized peptide derived from human p70 S6 kinase α around the phosphorylation site of Ser418
Swissprot
P23443
Synonyms
70 kDa ribosomal protein S6 kinase 1KS6B1p70 alphaP70 beta 1p70 ribosomal S6 kinase alphap70 ribosomal S6 kinase beta 1p70 S6 kinase alphaP70 S6 Kinasep70 S6 kinasealpha 1p70 S6 kinasealpha 2p70 S6Kp70 S6K-alphap70 S6KAp70(S6K) alphap70(S6
Calculated MW
59 kDa
Observed MW
60 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.