Phospho-SMAD2/3 (Thr8) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85953A
Western Blot analysis of VEC cells with Phospho-Smad2/3 (Thr8) Polyclonal Antibody
Product Name
Phospho-SMAD2/3 (Thr8) Polyclonal Antibody
Catalog Number
RD85953A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
Immunogen Information
Immunogen
Synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8
Swissprot
Q15796,P84022
Synonyms
SMAD2MADH2MADR2Mothers against decapentaplegic homolog 2MAD homolog 2Mothers against DPP homolog 2JV18-1Mad-related protein 2hMAD-2SMAD family member 2SMAD 2Smad2hSMAD2SMAD3MADH3Mothers against decapentaplegic
Calculated MW
48 kDa
Observed MW
48 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, ELISA 1:10000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.