PIK3C2A Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89422A
Western blot analysis of extracts of various cell lines using PIK3C2A Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse heart using PIK3C2A Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat heart using PIK3C2A Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver damage using PIK3C2A Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using PIK3C2A Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3 ug PIK3C2A Polyclonal Antibody. Western blot was performed from the immunoprecipitate using PIK3C2A Polyclonal Antibody at a dilution of 1:1000.
Product Name
PIK3C2A Polyclonal Antibody
Catalog Number
RD89422A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.
Immunogen Information
Immunogen
Recombinant fusion protein of human PIK3C2A
Gene ID
5286
Swissprot
O00443
Synonyms
PIK3C2ACPKPI3-K-C2(ALPHA)PI3-K-C2API3K-C2-alphaPI3K-C2alphaphosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Calculated MW
65kDa/69kDa/70kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.