PIWIL4 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89545A
Western blot analysis of extracts of various cell lines using PIWIL4 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat testis using PIWIL4 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using PIWIL4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
PIWIL4 Polyclonal Antibody
Catalog Number
RD89545A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).
Immunogen Information
Immunogen
Recombinant fusion protein of human PIWIL4
Gene ID
143689
Swissprot
Q7Z3Z4
Synonyms
PIWIL4HIWI2MIWI2
Calculated MW
52kDa
Observed MW
53kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas