PKC zeta Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD71661A
Western Blot analysis of COS7 cells treated with PMA (125ng/ml, 30mins) minus immunising peptide using PKC zeta Polyclonal Antibody at dilution of 1:2000.
Product Name
PKC zeta Polyclonal Antibody
Catalog Number
RD71661A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
Immunogen Information
Immunogen
Synthesized peptide derived from human PKC ζ around the non-phosphorylation site of Thr560.
Swissprot
Q05513
Synonyms
14-3-3-zetaisoformAI098070aPKCzetaC80388EC 2.7.11.13KPCZnPKC zetanPKC-zetaOTTHUMP00000001368OTTHUMP00000044160PKC 2PKC ZETAPKC2PkczPKCZETAPKM-zetaincludedPRKCZProtein kinase C zetaProtein kinase C zeta formProtein kinase C zeta typer1
Calculated MW
68 kDa
Observed MW
80 kDa
Applications
Reactivity
Human,Mouse,Rat,Monkey
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas