For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87198A
Western blot analysis of extracts of various cell lines using PLC beta 3 Polyclonal Antibody at1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using PLC beta 3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human PLC beta 3
Gene ID
5331
Swissprot
Q01970
Synonyms
PLCB3
Calculated MW
55kDa/58kDa
Observed MW
58KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.