For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90389A
Western blot analysis of extracts of various cell lines using PLZF Polyclonal Antibody at 1:2000 dilution.
Immunohistochemistry of paraffin-embedded mouse brain using PLZF Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat testis using PLZF Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.
Immunogen Information
Immunogen
Recombinant fusion protein of human PLZF
Gene ID
7704
Swissprot
Q05516
Synonyms
ZBTB16PLZFZNF145
Calculated MW
34kDa
Observed MW
36kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000,IHC 1:50-1:100
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.