POLD3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD84376A
Western blot analysis of extracts of various cell lines using POLD3 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human oophoroma using POLD3 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLD3 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using POLD3 Polyclonal Antibody
Product Name
POLD3 Polyclonal Antibody
Catalog Number
RD84376A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human POLD3 (NP_006582.1).
Gene ID
10714
Swissprot
Q15054
Synonyms
POLD3P66P68PPP1R128
Calculated MW
39 kDa/46 kDa/51 kDa
Observed MW
51 kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas