PPAR gamma Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86736A
Immunohistochemistry of paraffin-embedded mouse heart using PPARγ Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse liver using PPARγ Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using PPARγ Polyclonal antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
PPAR gamma Polyclonal Antibody
Catalog Number
RD86736A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
Immunogen Information
Immunogen
A synthetic peptide of human PPAR gamma
Gene ID
5468
Swissprot
P37231
Synonyms
CIMT1GLM1NR1C3PPARG1PPARG2PPARgammaPPAR gammaPPARG
Calculated MW
25kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
IHC,IF
Conjugation
Unconjugated
Dilution
IHC 1:50-1:200,IF 1:50-1:20
Concentration
1 mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.