PPL Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87775A
Western blot analysis of extracts of various cell lines using PPL Polyclonal Antibody at1:1000 dilution.
Immunofluorescence analysis of human skin cells using PPL Polyclonal Antibody at dilution of 1:500 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse skin cells using PPL Polyclonal Antibody at dilution of 1:500 (40x lens). Blue: DAPI for nuclear staining.
Product Name
PPL Polyclonal Antibody
Catalog Number
RD87775A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.
Immunogen Information
Immunogen
Recombinant fusion protein of human PPL
Gene ID
5493
Swissprot
O60437
Synonyms
PPL
Calculated MW
62kDa
Observed MW
62kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:20
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.