PRPF3 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD83333A
Western blot analysis of extracts of various cell lines using PRPF3 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using PRPF3 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat brain using PRPF3 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using PRPF3 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human stomach using PRPF3 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human stomach using PRPF3 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using PRPF3 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using PRPF3 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PRPF3 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using PRPF3 Polyclonal Antibody
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Overview

Circle
Product Name
PRPF3 Polyclonal Antibody
Catalog Number
RD83333A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human PRPF3 (NP_004689.1).
Gene ID
9129
Swissprot
O43395
Synonyms
PRPF3HPRP3HPRP3PPRP3Prp3pRP18SNRNP90
Calculated MW
31 kDa/77 kDa
Observed MW
100 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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