PRPF8 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD83617A
Western blot analysis of extracts of various cell lines using PRPF8 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human esophagus using PRPF8 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using PRPF8 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse stomach using PRPF8 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using PRPF8 Polyclonal Antibody at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.
Product Name
PRPF8 Polyclonal Antibody
Catalog Number
RD83617A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
Immunogen Information
Immunogen
A synthetic peptide of human PRPF8 (NP_006436.3).
Gene ID
10594
Swissprot
Q6P2Q9
Synonyms
PRPF8HPRP8PRP8PRPC8RP13SNRNP220PRPF8
Calculated MW
273 kDa
Observed MW
274 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas