PSMB3 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using PSMB3 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using PSMB3 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat lung using PSMB3 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat lung using PSMB3 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human liver cancer using PSMB3 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human liver cancer using PSMB3 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using PSMB3 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using PSMB3 Polyclonal Antibody at dilution of 1:100 (40x lens).
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
PSMB3 Polyclonal Antibody
Catalog Number
RD85427A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The 26 S proteasome may be involved in trinucleotide repeat expansion, a phenomenon which is associated with many hereditary neurological diseases. Pseudogenes have been identified on chromosomes 2 and 12. Alternative splicing results in multiple transcript variants

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human PSMB3 (NP_002786.2).
Gene ID
5691
Swissprot
P49720
Synonyms
PSMB3HC10-II
Calculated MW
22 kDa
Observed MW
23 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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