For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89141A
Western blot analysis of extracts of MCF7 cells using PTGES Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using PTGES Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Rat testis using PTGES Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse testis using PTGES Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
The protein encoded by this gene is a glutathione-dependent prostaglandin E synthase. The expression of this gene has been shown to be induced by proinflammatory cytokine interleukin 1 beta (IL1B). Its expression can also be induced by tumor suppressor protein TP53, and may be involved in TP53 induced apoptosis. Knockout studies in mice suggest that this gene may contribute to the pathogenesis of collagen-induced arthritis and mediate acute pain during inflammatory responses.
Immunogen Information
Immunogen
A synthetic peptide of human PTGES
Gene ID
9536
Swissprot
O14684
Synonyms
PTGESMGST-IVMGST1-L1MGST1L1MPGESPGESPIG12PP102PP1294TP53I12mPGES-1prostaglandin E synthase
Calculated MW
69kDa/81kDa/89kDa/96kDa/132kDa
Observed MW
126KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.