Rad21 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90253A
Western blot analysis of extracts of various cell lines using Rad21 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of C6 cells using Rad21 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using Rad21 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Rad21 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using Rad21 Polyclonal Antibody at a dilution of 1:1000.
Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Rad21 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using Rad21 Polyclonal Antibody at a dilution of 1:1000.
Product Name
Rad21 Polyclonal Antibody
Catalog Number
RD90253A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]
Immunogen Information
Immunogen
Recombinant fusion protein of human Rad21
Gene ID
5885
Swissprot
O60216
Synonyms
CDLS4HR21HRAD21MCD1NXP1SCC1hHR21
Observed MW
30KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.