Rad23B Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89430A
Western blot analysis of extracts of various cell lines using Rad23B Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon using Rad23B Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse testis using Rad23B Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using Rad23B Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
Rad23B Polyclonal Antibody
Catalog Number
RD89430A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Immunogen Information
Immunogen
A synthetic peptide of human Rad23B
Gene ID
5887
Swissprot
P54727
Synonyms
RAD23BHHR23BHR23BP58UV excision repair protein RAD23 homolog B
Calculated MW
33kDa/38kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas