RAD50 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD76415A
Western Blot analysis of 293T, Hela, K562, NIH/3T3 and RAW264.7 cell using RAD50 Polyclonal Antibody at dilution of 1:900
Immunohistochemistry of paraffin-embedded Human colon cancer using RAD50 Polyclonal Antibody at dilution of 1:40
Immunohistochemistry of paraffin-embedded Human gastric cancer using RAD50 Polyclonal Antibody at dilution of 1:40
Product Name
RAD50 Polyclonal Antibody
Catalog Number
RD76415A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Immunogen Information
Immunogen
Synthetic peptide of human RAD50
Swissprot
Q92878
Synonyms
DNA repair protein RAD50hRAD50hsRAD50MrellNBSLDRad 50RAD50 2rad50RAD50 homolog (S cerevisiae)RAD50 homologRAD50 PENRAD50S. cerevisiaehomolog ofRAD50RAD502Rad50lTruncated RAD50 protein
Calculated MW
154 kDa
Gene Accession
NP_005723
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:200
Concentration
0.4 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas