RCN1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89431A
Western blot analysis of extracts of various cell lines using RCN1 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of L929 cells using RCN1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using RCN1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
RCN1 Polyclonal Antibody
Catalog Number
RD89431A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.
Immunogen Information
Immunogen
Recombinant fusion protein of human RCN1
Gene ID
5954
Swissprot
Q15293
Synonyms
RCN1HEL-S-84PIG20RCALRCNreticulocalbin-1
Calculated MW
80kDa
Observed MW
Refer to figures
Applications
Reactivity
Human
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.