Recombinant Acetyl-Histone H3 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86997A
Western blot detection of Histone H3 (acetyl-Lys27) in Hela,A549,HL-60 cell lysates using Histone H3 (acetyl-Lys27) Rabbit mAb(1:1000 diluted).Predicted band size:15kDa.Observed band size:17kDa.
Immunohistochemistry of Histone H3 (acetyl-Lys27) in paraffin-embedded Human lung cancer tissue using Histone H3 (acetyl-Lys27) Rabbit mAb at dilution 1:50
Immunofluorescence of Histone H3 (acetyl-Lys27)(green) in hela using Histone H3 (acetyl-Lys27) Rabbit mAb at dilution 1:50, and DAPI(blue)
Product Name
Recombinant Acetyl-Histone H3 Monoclonal Antibody
Catalog Number
RD86997A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Immunogen Information
Immunogen
A synthetic acetyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
Gene ID
8290
Swissprot
P68431
Synonyms
H3 histone familymember AH3/AH31H3FAHist1h3aHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3Jhistone 1H3aHistone cluster 1H3aHistone H3.1Histone H3/aHistone H3/bHistone H3/cHistone H3/dHistone H3/fHistone H3
Calculated MW
74kDa
Observed MW
74/48kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas