Recombinant AKT Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86928A
Western blot detection of AKT1/2/3 in Jurkat,Rat Brain,C6,CHO-K1,Hela cell lysates using AKT Rabbit mAb(1:1000 diluted).Predicted band size:56kDa.Observed band size:56kDa.
Immunohistochemistry of AKT1/2/3 in paraffin-embedded Human lung cancer tissue using AKT1/2/3 Rabbit mAb at dilution 1:50
Product Name
Recombinant AKT Monoclonal Antibody
Catalog Number
RD86928A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Immunogen Information
Immunogen
Recombinant protein of human AKT1/2/3
Gene ID
10000
Swissprot
P31749/P31751/Q9Y243
Synonyms
PKBRACPRKBAPKB-ALPHARAC-ALPHAAKT1AKT2AKT3
Calculated MW
96kDa
Observed MW
96kDa
Applications
Reactivity
Human,Mouse,Rat,Hamster
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.