Recombinant alpha Smooth Muscle Actin Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86930A
Western blot detection of alpha smooth muscle Actin in Rat Brain,C6,3T3,Hela cell lysates using alpha smooth muscle Actin Rabbit mAb(1:1000 diluted).Predicted band size:42kDa.Observed band size:42kDa.
Immunohistochemistry of alpha smooth muscle Actin in paraffin-embedded Human colon cancer tissue using alpha smooth muscle Actin Rabbit mAb at dilution 1:100
Product Name
Recombinant alpha Smooth Muscle Actin Monoclonal Antibody
Catalog Number
RD86930A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Immunogen Information
Immunogen
A synthetic peptide of human alpha smooth muscle Actin
Gene ID
59
Swissprot
P62736
Synonyms
a actinAAT6ACTAACTA2Actin alpha 2 smooth muscle aortaActin aortic smooth muscleActinaortic smooth muscleACTSAACTVSAlpha 2 actinAlpha actin 2Alpha cardiac actinalpha smaAlpha-actin-2Cell growth inhibiting gene 46 proteinCell growth-inhibiti
Calculated MW
50kDa
Observed MW
50kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.