Recombinant alpha Tubulin Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86931A
Western blot detection of alpha Tubulin in Hela,CHO-K1,Rat Brain lysates using alpha Tubulin Rabbit mAb(1:500 diluted).Predicted band size:50kDa.Observed band size:50kDa.
Immunohistochemistry of alpha Tubulin in paraffin-embedded Human tonsil using alpha Tubulin Rabbit mAb at dilution 1:50
Immunofluorescence of alpha Tubulin(green) in hela using alpha Tubulin Rabbit mAb at dilution 1:50, and DAPI(blue)
Product Name
Recombinant alpha Tubulin Monoclonal Antibody
Catalog Number
RD86931A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.MiscellaneousThis tubulin does not have a C-terminal tyrosine.RegionsFeature keyPosition(s)Description
Immunogen Information
Immunogen
A synthetic peptide of human alpha Tubulin
Gene ID
7277
Swissprot
P68366
Synonyms
ALS22TUBA1H2-ALPHA
Calculated MW
33kDa
Observed MW
55kDa
Applications
Reactivity
Human,Mouse,Rat,Hamster
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.