Recombinant APG5L Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86932A
Western blot detection of APG5L/ATG5 Rabbit pA in K562,C6,Hela cell lysates using APG5L/ATG5 Rabbit mAb(1:1000 diluted).Predicted band size:33kDa.Observed band size:55kDa.
Immunofluorescence of APG5L/ATG5 (green) in MCF-7 using APG5L/ATG5 antibody at dilution 1:20, and DAPI(blue)
Product Name
Recombinant APG5L Monoclonal Antibody
Catalog Number
RD86932A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
ATG5,also named as APG5L and ASP,belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process,possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process,occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection.
Immunogen Information
Immunogen
A synthetic peptide of human APG5L/ATG5
Gene ID
9474
Swissprot
Q9H1Y0
Synonyms
APG 5APG 5LAPG5APG5 autophagy 5 likeAPG5 likeAPG5-likeAPG5LApoptosis specific proteinApoptosis-specific proteinASPATG 5Atg5ATG5 autophagy related 5 homologATG5Autophagy protein 5Autophagy related 5hAPG5Homolog of S Cerevisiae autophagy 5
Calculated MW
119kDa
Observed MW
150kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IF 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.