For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86933A
Western blot detection of BAT3/BAG-6 in K562,Rat Brain,C6,3T3 cell lysates using BAT3/BAG-6 Rabbit mAb(1:1000 diluted).Predicted band size:119kDa.Observed band size:150kDa.
Immunofluorescence of BAT3/BAG-6 (green) in MCF-7 using BAT3/BAG-6 antibody at dilution 1:20, and DAPI(blue)
This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human BAT3
Gene ID
7917
Swissprot
P46379
Synonyms
BAG6BAG-6BAT3D6S52EG3
Calculated MW
43kDa
Observed MW
43/38kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IF 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.