Recombinant Bax Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot detection of Bax in C6,3T3,Hela cell lysates using Bax Rabbit mAb(1:1000 diluted).Predicted band size:21kDa.Observed band size:20kDa.
Immunohistochemistry of Bax in paraffin-embedded Human lung cancer tissue using Bax Rabbit mAb at dilution 1:20
Product Name
Recombinant Bax Monoclonal Antibody
Catalog Number
RD86906A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
BAX,also named as BCL2L4,is a pro-apoptotic member of the Bcl-2 protein family,which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells,but accumulates on the outer mitochondrial membrane upon apoptosis induction . BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane,which leads to the release of cytochrome c from mitochondria . The expression of BAX is upregulated by the tumor suppressor protein p53,and BAX has been shown to be involved in p53-mediated apoptosis .
Immunogen Information
Immunogen
A synthetic peptide of human Bax
Gene ID
581
Swissprot
Q07812
Synonyms
Apoptosis regulator BAXBAXBax-proteinBAXBAXABaxdelta2G9Baxdelta2G9omegaBaxdelta2omegaBcl-2-like protein 4BCL2 associated X proteinBCL2 associated X protein omegaBCL2 associated X protein transcript variant delta2Bcl2-L-4BCL2L4membrane isofor
Calculated MW
55kDa
Observed MW
55kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas