Recombinant Bcl2 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86898A
Western blot detection of Bcl-2 in Jurkat,Hela cell lysates using Bcl-2 Rabbit mAb(1:1000 diluted).Predicted band size:26kDa.Observed band size:26kDa.
Immunohistochemistry of Bcl-2 in paraffin-embedded Human breast cancer tissue using Bcl-2 Rabbit mAb at dilution 1:100
Product Name
Recombinant Bcl2 Monoclonal Antibody
Catalog Number
RD86898A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Immunogen Information
Immunogen
A synthetic peptide of human Bcl-2
Gene ID
596
Swissprot
P10415
Synonyms
Apoptosis regulator Bcl 2Apoptosis regulator Bcl-2Apoptosis regulator Bcl2AW986256B cell CLL/lymphoma 2B cell leukemia/lymphoma 2Bcl-2Bcl2BCL2C430015F12RikD630044D05RikD830018M01RikLeukemia/lymphomaB-cell2Oncogene B-cell leukemia 2PPP1R50
Calculated MW
34kDa
Observed MW
34kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:20-1:200
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas