Recombinant Bcr Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87008A
Western blot detection of Bcr in A549,HL-60,U2OS,C6 cell lysates using Bcr Rabbit mAb(1:1000 diluted).Predicted band size:143kDa.Observed band size:160kDa.
Immunohistochemistry of Bcr in paraffin-embedded Human Brain using Bcr Rabbit mAb at dilution 1:50
Product Name
Recombinant Bcr Monoclonal Antibody
Catalog Number
RD87008A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human Bcr
Gene ID
613
Swissprot
P11274
Synonyms
ALLbcrBCR1Breakpoint cluster regionCMLD22S11D22S662PHLRenal carcinoma antigen NY-REN-26
Calculated MW
22kDa
Observed MW
22kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas