Recombinant c Met Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87010A
Western blot detection of Met (c-Met) in K562,C6,3T3 cell lysates using Met (c-Met) Rabbit mAb(1:1000 diluted).Predicted band size:156kDa.Observed band size:170kDa.
Immunohistochemistry of Met (c-Met) in paraffin-embedded Human lung cancer tissue using Met (c-Met) Rabbit mAb at dilution 1:50
Immunofluorescence of Met (c-Met)(green) in Hela cells using Met (c-Met) Rabbit mAb at dilution 1:200, and DAPI(blue)
Product Name
Recombinant c Met Monoclonal Antibody
Catalog Number
RD87010A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human Met (c-Met)
Gene ID
4233
Swissprot
P08581
Synonyms
METAUTS9DFNB97HGFRRCCP2c-Met
Calculated MW
59kDa
Observed MW
59kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:20-1:50, IF 1:20-1:200
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas