Recombinant c Myc Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot detection of c-Myc in K562,C6,3T3,Hela cell lysates using c-Myc Rabbit mAb(1:1000 diluted).Predicted band size:49kDa.Observed band size: 57-65kDa.
Western blot detection of c-Myc in K562,C6,3T3,Hela cell lysates using c-Myc Rabbit mAb(1:1000 diluted).Predicted band size:49kDa.Observed band size: 57-65kDa.
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Overview

Circle
Product Name
Recombinant c Myc Monoclonal Antibody
Catalog Number
RD86916A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

Immunogen Information

Circle
Immunogen
A synthetic peptide of human c-Myc
Gene ID
4609
Swissprot
P01106
Synonyms
AU016757Avian myelocytomatosis viral oncogene homologbHLHe39c MycClass E basic helix-loop-helix protein 39MRTLMycMyc proteinMyc proto oncogene proteinMyc proto-oncogene proteinmyc-related translation/localization regulatory factorMYCMyc2MYCC
Calculated MW
32kDa
Observed MW
32kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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