Recombinant Caspase 9 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87014A
Western blot detection of Caspase-9 in 3T3,Hela cell lysates using Caspase-9 Rabbit mAb(1:1000 diluted).Predicted band size:46kDa.Observed band size:46kDa.
Product Name
Recombinant Caspase 9 Monoclonal Antibody
Catalog Number
RD87014A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Caspase 9,apoptosis-related cysteine protease (CASP9,synonyms: MCH6,APAF3,APAF-3,ICE-LAP6,CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits,large and small,that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
Immunogen Information
Immunogen
Recombinant protein of human Caspase-9
Gene ID
842
Swissprot
P55211
Synonyms
APAF-3APAF3Apoptosis related cysteine peptidaseApoptotic protease Mch-6Apoptotic protease-activating factor 3CASP-9CASP9CASP9Caspase 9 apoptosis related cysteine peptidaseCaspase 9 Dominant NegativeCaspase 9cCaspase-9Caspase-9 subunit p10ICE
Calculated MW
60kDa
Observed MW
60kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas