Western blot detection of Cathepsin B in Rat Brain,C6,3T3 cell lysates using Cathepsin B Rabbit mAb(1:1000 diluted).Predicted band size:37kDa.Observed band size: 27,30kDa.
Immunofluorescence of Cathepsin B (green) in Raw264.7 using Cathepsin B antibody at dilution 1:50, and DAPI(blue)
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Has also been implicated in tumor invasion and metastasis.
Immunogen Information
Immunogen
Recombinant protein of mouse Cathepsin B
Gene ID
13030
Swissprot
P10605
Synonyms
CB
Calculated MW
20kDa
Observed MW
20kDa
Applications
Reactivity
Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.