Recombinant CCT2 Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86971A
Western blot detection of CCT2 in Hela,A549,HL-60,U2OS,U87-MG cell lysates using CCT2 Rabbit mAb(1:1000 diluted).Predicted band size:57kDa.Observed band size:57kDa.
Western blot detection of CCT2 in Hela,A549,HL-60,U2OS,U87-MG cell lysates using CCT2 Rabbit mAb(1:1000 diluted).Predicted band size:57kDa.Observed band size:57kDa.
Immunohistochemistry of CCT2 in paraffin-embedded Human lung cancer tissue using CCT2 Rabbit mAb at dilution 1:50
Immunohistochemistry of CCT2 in paraffin-embedded Human lung cancer tissue using CCT2 Rabbit mAb at dilution 1:50
Immunofluorescence of CCT2(green) in hela using CCT2 Rabbit mAb at dilution 1:50, and DAPI(blue)
Immunofluorescence of CCT2(green) in hela using CCT2 Rabbit mAb at dilution 1:50, and DAPI(blue)
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
Recombinant CCT2 Monoclonal Antibody
Catalog Number
RD86971A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

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Immunogen
A synthetic peptide of human CCT2
Gene ID
10576
Swissprot
P78371
Synonyms
CCT299D8.1CCT-betaCCTBHEL-S-100nPRO1633TCP-1-beta
Calculated MW
139kDa
Observed MW
139kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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