Recombinant Cofilin Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87021A
Western blot detection of Cofilin in A549,MCF-7,293T cell lysates using Cofilin Rabbit mAb(1:1000 diluted).Predicted band size:19kDa.Observed band size:19kDa.
Immunohistochemical of Cofilin in Human lung cancer tissue using Cofilin antibody at dilution 1:200
Immunofluorescence of Cofilin(green) in hela using Cofilin Rabbit mAb at dilution 1:50, and DAPI(blue)
Product Name
Recombinant Cofilin Monoclonal Antibody
Catalog Number
RD87021A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility,cytokinesis,and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity,also causing translocation from the nucleus to the cytoplasm.
Immunogen Information
Immunogen
A synthetic peptide of human Cofilin
Gene ID
1072
Swissprot
P23528
Synonyms
18 kDa phosphoproteinCFL 1CFLCFL1COF1Cofilin 1Cofilin 1 non muscleCofilinCofilin non muscle isoformCofilin-1epididymis secretory protein Li 15HEL-S-15non-muscle isoformp18
Calculated MW
73kDa
Observed MW
80kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:200, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas