Recombinant Collagen III alpha 1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86936A
Western blot detection of Collagen III in K562,C6,3T3 cell lysates using Collagen III Rabbit mAb(1:1000 diluted).Predicted band size:139kDa.Observed band size:150kDa.
Immunofluorescence of Collagen III(green) in Hela cells using Collagen III Rabbit mAb at dilution 1:50, and DAPI(blue)
Variation
Quantity
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Overview
Product Name
Recombinant Collagen III alpha 1 Monoclonal Antibody
Catalog Number
RD86936A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Immunogen Information
Immunogen
Recombinant protein of human Collagen III
Gene ID
1281
Swissprot
P02461
Synonyms
Alpha 1 type III collagenAlpha1 (III) collagenCO3A1COL 3A1COL3A1Collagen alpha 1(III) chainCollagen alpha-1(III) chainCollagen III alpha 1 chain precursorCollagen III alpha 1 polypeptideCollagen type III alpha 1 (Ehlers Danlos syndrome type IV au
Calculated MW
69kDa
Observed MW
69kDa
Applications
Reactivity
Human
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IF 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas