Western blot detection of TORC2 in A549,HL-60,U251 cell lysates using TORC2 Rabbit mAb(1:1000 diluted).Predicted band size:73kDa.Observed band size:80kDa.
Immunohistochemistry of TORC2 in paraffin-embedded Human breast cancer tissue using TORC2 Rabbit mAb at dilution 1:50
This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm.
Immunogen Information
Immunogen
A synthetic peptide of human TORC2
Gene ID
200186
Swissprot
Q53ET0
Synonyms
CREB regulated transcription coactivator 2CREB-regulated transcription coactivator 2CRTC2CRTC2RP11-422P24.6TORC-2torc2Transducer of CREB protein 2Transducer of regulated cAMP response element-binding proteinTransducer of regulated cAMP response e
Calculated MW
47kDa
Observed MW
47kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.